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These were allowed to adhere overnight in full culture media (DMEM (41965039) with 4 mM L-glutamine, 10% dFBS (26400044), 1 mM sodium pyruvate (11360039) and 50 U ml 1 penicillinstreptomycin (15070063)) under normal cell culture conditions

Next, the mixture was sifted through a 400-m sieve and mixed well with water (250 ml/kg) to produce a stiff dough

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