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On day 4, after 48 h of pretreatment, the medium was removed and replaced with medium containing lethal compounds or other modulating compounds (such as BSO [100 M])

The regimen depends on medical supervision, patient tolerance, and safety monitoring

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Since only the lipid-soluble and non-ionized, part of VPA propagates across membranes, only a limited amount of VPA is transported into tissues via passive diffusion (Zobdeh et al., 2021)