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Immunocytochemistry Immunolabeling of NPCs and derived neurons was accomplished by utilization of a standard two-day protocol with: 15 min fixation in 4% PFA, 10 min permeabilization in 0.1% triton-PBS and 30 min blocking with 5% donkey serum

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autoimmune thyroid diseases [14]) and, more generally, in approximately 10% of the general population [15]

To enhance the efficiency of acclimatization, various substances and preparations that positively affect plant processes and, consequently, improve the condition and survival of plants, are also used during both in vitro propagation and acclimatization stages

It is found primarily in meat