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For immunoblotting, 20 g per specimen was separated on 420% gradient precast gels (Mini-PROTEAN TGX, Bio-Rad) and transferred to polyvinylidene difluoride membranes (Immobilon -P, Merck Millipore)

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doi: 10.1016/0003-2697(76)90527-3

Graves disease affects the thyroid gland