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Acknowledgements The authors would like to thank the experimental apparatus and technical support provided by the Key Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine

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The primary benefits associated with vitamin B12 shots may include an increase in energy levels, improvement in mood, and a reduction in symptoms of depression and anxiety levels, which are often associated with B12 deficiency

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Hormonal conditions such as hypothyroidism or PCOS may also influence the rate of weight loss and should be assessed by a clinician