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Some protocols report improved lipid profiles (lower triglycerides, improved HDL/LDL ratio) while others report minimal changes

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When separated into tertiles of AST and ALT, triple therapy promoted significant reductions in ALT and AST compared to baseline and conventional therapy

Cappelli C, Rotondi M, Pirola I, Agosti B, Formenti AM, De Cata P, Salvetti M, Chiovato L, Castellano M

Core analytical requirements include: Primary structure confirmation using LC-MS/MS to verify intact mass, sequence integrity and detect modifications Impurity profiling (e.g., sequence variants, degradation products) using high-resolution techniques such as UHPLC-HRMS Aggregation and molecular weight analysis using orthogonal methods like SEC-MALS and sedimentation velocity analytical ultracentrifugation (SV-AUC)