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After three brief washes with PBS, the samples were incubated with 10 mM MgCl 2 in PBS for a further 5 minutes and then mounted on a well slide in freshly prepared GLOX buffer (100 mM Tris-HCl pH 8, 10% w/v glucose, 0.4 mg/mL glucose oxidase, 64 g/mL catalase) supplemented with 10 mM MEA and sealed with two part silicon (eco-sil speed, Picodent)

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