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The direct iron-independent bactericidal action of Lf was discovered in 1977 when it was observed that human Lf inhibited the growth of Vibrio cholerae and Streptococcus mutans (upon contact with an iron-rich medium), but that this bacteriostatic activity did not regress upon addition of iron

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Similarly, a randomised control trial in older adults with median age 73 years reported that supplementation of 500 mcg vitamin B12, 400 mcg folic acid and 15 mcg vitamin D3 for two years resulted in an average reduction of homocysteine by approximately -4.2 micro mol/L compared to the placebo group (De Koning et al, 2016)

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This finding accounts for the important observation that the c-met binding affinity of LASSS/peroxynitrite-treated HGF (at the 1:8000:2000 molar ratio to HGF, with the wash-out of un-reacted free LASSS) was significantly higher than the original level of un-treated positive control HGF (Figure 2d, bars 2 and 6 )