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However, a few controlled studies suggest that vitamin K supplements benefit bone and heart health

CTK solution (0.25% trypsin + 1 mg/mL collagenase IV + 1 mM CaCl 2 + 20% KSR in DPBS ()) was prepared with trypsin ((2.5%), no phenol red, Thermo Fisher Scientific Inc.), collagenase (type IV, powder, Thermo Fisher Scientific Inc.), calcium chloride dihydrate (Fujifilm Wako Pure Chemical Corporation), Knock Out TM Serum Replacement (KSR, Thermo Fisher Scientific Inc.)

Studies confirm that ion release (Ca 2+ , F , PO 4 ) at acidic pH (4.5-5.5) is markedly higher than at neutral pH (6.5), a trend sustained even after recharging (Venkataiah et al., 2025)

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