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The supernatant (150 L) was placed in a clear flat-bottom 96-well plate in duplicate and absorbance measured at 620 nm in a Biotek Synergy HT plate reader

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To identify which syndrome was prevalent in mice, we used PBI (8.5 Gy), sparing 510% of the mouse bone marrow from irradiation, thus precluding the development of severe H-ARS

MMNST is an extremely rare entity