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Statistics and reproducibility The experiments including TEM observation, western blot analysis, immunohistochemical assessments of GGT and CD8 + T cells expression, two-photon CLSM examination, dual staining with Calcein-AM and PI for viability analysis, histological analysis of tumor tissues, and CLSM-based visualization of nanoprobe internalization by tumor cells and 3D cell spheroids, intracellular ROS generation, nanoprobe distribution in brain tissue sections, alterations in mitochondrial membrane potential, and CALR exposure in tumor cells were repeated three times

10.1248/bpb.b13-00469 315 Yoon HeeraY

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