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The procedure involved obtaining red blood cells by centrifuging mouse blood at 2,000 rpm for 5 min, followed by washing them with PBS three times

Extensive vascular remodeling was coupled to altered increased production of F2-isoprostane, 8-epi-prostaglandin F2 alpha, nuclear localization of NFkappaB and altered glutathione homeostasis

Assay: Glutathione in human cells

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High ROS levels induce reversible oxidative PTMs on critical thiol residues of ETC proteins (for example S-glutathionylation or S-nitrosylation of Complex I subunits), impairing electron transport efficiency and increasing electron leakage that further amplifies ROS production and lowers m (58)