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Notably, melatonin mediates the nuclear output of PGC1 by upregulating the expression of SIRT1 and increases the expression of mitochondrial biogenesis markers PGC1 and TFAM after melatonin treatment, thereby enhancing autophagy and mitochondrial health (Siddhi et al., 2022)

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however, calorie-dense liquids can slow gastric emptying due to interactions between nutrients and intestinal receptors

Sections were microwave treated for 8 min at 900 W and 30 min at 360 W in Tris-EDTA buffer, pH 9.0, were allowed to cool for 15 min, rinsed in Tris-buffered saline (TBS), pH 7.5, and blocked in 3% H 2 O 2 with 0.15 M NaN 3