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The concentration of delta sleep-inducing peptide (DSIP) in the samples can be calculated by comparing the OD of the samples to a standard curve

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FIGURE 4 Validation of RNA-seq results and the expression analysis of Wnt/-catenin signaling pathway-related genes To verify the results of RNA-seq, 13 differentially expressed genes, including 6 downregulated and 7 upregulated genes, were randomly selected for qRT-PCR assay