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The washed cell suspension was inoculated into 49 ml pre-warmed DRM supplemented with carbenicillin, spectinomycin and 20 M arabinose in a disposable 125-ml bacterial culture flask

The most common vitamin B12 deficiency symptoms are: Fatigue Weakness Loss of appetite Weight loss Numbness and tingling in the hands and feet Soreness of the mouth or tongue A pale yellow tinge to your skin Difficulty maintaining balance Disturbed vision Depression Confusion Poor short-term memory A severe vitamin B12 deficiency can cause megaloblastic anemia, also known as vitamin B12 deficiency anemia, where red blood cells are not produced properly and are larger than normal