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The obtained supernatant was filtered through a nylon membrane with a pore size of 0.22 m, and it was injected into HPLC (Waters) equipped with a reversed phase column (250 mm 4.6 mm) Waters Spherisorb ODS-2 and 5 m particle size, coupled to an electrochemical detector (DECADE) fitted with a working carbon electrode and with a reference hydrogen electrode at 520 mV and with UV/Vis detector at 216 nm to detect GSSG

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Oral glutathione unless liposomal or acetylated is broken down by peptidases in the intestine, with less than 10% reaching systemic circulation

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