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After incubation at 30 C for 16 h, the subsequent sample preparation and LCQTOF or LCMS (Agilent LCMSD iQ) analysis followed the same methods as described for the Cp1B reaction

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Published studies have shown that NNMT expression affects myogenic differentiation the process by which satellite cells activate, proliferate, and fuse to form new muscle fibers

Meanwhile, the alternative loss recoupment limit is not expected to materially reduce shared losses collected by the program as only a few ACOs have shared losses, and those losses rarely approach the regular benchmark-based loss recoupment limit