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Phosphate-Buffered Saline (PBS): Provides pH buffering, which is critical for maintaining the ionization state of the peptides amino acid side chains, ensuring the 3D conformation for receptor binding is maintained

Mechanisms include: UVB: Direct DNA damage (CPDs) that impair fibroblast function UVA: Oxidative stress that degrades collagen and elastin Anatomical reflection: Light bounces into the orbital area, increasing cumulative exposure This is why we stress sunscreen around the eye area is not optional

The primer sequences of the target and control genes were as follows: Il6 forward: 5-ACA AAG CCA GAG TCC TTC AGA G-3 and reverse, 5-GGT CCT TAG CCA CTC CTT CTG-3