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doi: 10.1007/s10930-021-09974-9

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These parameters may be evaluated using tumor biopsy specimens and patient-derived samples through a combination of complementary experimental and clinical methodologies, including immunohistochemistry or spatial proteomics to assess FSP1 expression and localization, metabolomics or lipidomics-based omics approaches to quantify CoQ redox state and lipid peroxidation signatures and functional ferroptosis-sensitivity assays in patient-derived tumor models such as organoids or xenografts

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GSR utilizes NADPH, derived from the PPP (of which G6PD catalyzes the first committed step), for recycling oxidized glutathione disulfide to reduced glutathione (Fig