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Clinical applications of metabolite ratio analysis include use in newborn screening for some inherited disorders of amino acid and organic acid metabolism and of mitochondrial fatty acid beta-oxidation (35, 36)

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These results suggest that while the AS treatment initially enhances the O phase but later reduces the J, I, and P phases, the combined treatment (AS + FeNP) reverses this effect and maximizes fluorescence intensity and amplitude across all OJIP phases

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