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An in-depth analysis of the molecular mechanisms of ferroptosis reveals multiple potential therapeutic targets and diverse regulatory pathways, providing directions for novel therapeutic strategies in kidney disease treatment
iron contained in iron-sulfur clusters
After quantification of RNA with NanoDrop 2000 (Thermo Fisher), cDNA was synthesised by mixing RNA with FS buffer, DTT (Thermo Fisher), and Random Primers (Sigma-Aldrich) and incubated for 3 min at 70 C
123 Following promising preclinical findings, several clinical trials have been initiated to evaluate the neuroprotective efficacy of GLP-1RAs in diverse populations, including individuals with diabetes, those at risk of cognitive decline, and patients diagnosed with AD or PD