aCSF was removed by careful suctioning and replaced with aCSF containing vehicle (aCSF + aCSF), 100 ng/ml with carrier free adult recombinant Hepatocyte Growth Factor (HGF) (R and D Systems, MN, USA) in aCSF, 10 "10 M Hinge (Harding lab), 50 ng/ml in aCSF, 10 "10 M Dihexa (Harding lab) in aCSF, 10 "12 M Dihexa in aCSF or 50 ng/ml HGF + 10 " ' 2 M Dihexa in aCSF for 30 minutes at 37C with gentle rocking
For those in higher-risk categories (over 60, metformin users, vegans), additional B12 supplementation beyond the formulation may be warranted
Korkushko, Experimental Gerontology (2004) Khavinson, Neuroendocrinology Letters (2001) Shift work and heavy travel For people who experience repeated night shifts or jet lag, cycling Epitalon around these periods may support circadian recovery, much like scheduling maintenance after heavy stress
The oral capsule format is of particular interest in this area, since the swallowed peptide is delivered directly into the gastrointestinal tract, where much of this research activity has historically been localised and where the compounds reported stability in gastric conditions is most relevant
Safety and regulatory status differ sharply between these two ingredients, and understanding the distinction is important before choosing either
