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For BSO treatment, cells were GSH starved for 48 h before washing and addition of EV-F3 and further 0.4 mM BSO (Sigma)

Our data from real-time qPCR disclosed upregulated mRNA levels of Clec7a and Trem2 in cholesterol-enriched microglia exposed to both pathogen-associated molecules and A peptides, likely mirroring a microglial switch to damage/neurodegeneration-associated genetic signatures

Lifestyle compatibility Weekly injections require minimal lifestyle adjustment

NAC supplementation has been studied for heavy metal detoxification, with some evidence of benefit for mercury and lead excretion, though clinical trials are limited [37]

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