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Immunoblotting The dishes were washed 2 with ice-cold PBS, lysed in 1 Laemli sample buffer, and subjected to standard SDS-PAGE using separate 4%12% Bis-Tris gels, after which the proteins were overnight transferred at 4C to polyvinylidene fluoride membranes

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Dose reduction may work for select patients, but complete discontinuation remains difficult without alternative therapies or a multidisciplinary approach