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Through its modulation of DNA methylation, histone modifications, and non-coding RNAs, IF impacts individual health and extends its effects across generations to impact aging, metabolism, and longevity

Immunoblotting To profile protein lipoylation and lysine glutarylation, 0.5 10 6 HeLa cells or 1 10 6 T cells were lysed in SDS loading buffer (2% (wt/vol) SDS, 50 mM Tris (pH 7.4), 150 mM NaCl, 1 mM dithiothreitol, 10% (vol/vol) glycerol and 0.2% (vol/vol) benzonase nuclease (Sigma-Aldrich, E1014)) and heated at 90 C for 5 min

Acharya P, Mathur MC (2020) Oxidative stress in alopecia areata: a systematic review and meta-analysis

Varani, J

Biochem 214 , 829835 (1993)