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During the reduction of peroxidase by mammalian GPX1, the Sec active site needs to be modified to form an intermediate stable form (Trenz et al., 2021

M., Konishi, N., and Ostergaard, K

To initiate a peroxidase reaction, the sections were rinsed with a washing buffer and subsequently incubated with 3,3-diaminobenzidine tetrahydrochloride (DAB, Thermo Fisher Scientific, Rockford, IL, USA)

The role of ascorbate in programmed cell death (PCD) is related to its role in the control of the activation of the ROS signalling branch of innate immune responses (Pavet et al