Seeds were surface-sterilized and germinated on 1/2 Murashige and Skoog medium containing 2% sucrose and 0.8% Phytagar and grown in a 22C growth chamber with a 16-h light and 8-h dark photoperiod after vernalization at 4C for 3 days
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They play a crucial role in the processes of vesicle biogenesis and exocytosis, including membrane transport proteins (such as Rab GTPases), heat shock proteins (such as HSP70 and HSP90), and ESCRT-related proteins (such as Tsg101 and Alix) [71] (Fig