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Int J Clin Exp Med (2012) 5:20820

Under NCL conditions with SetCys peptides, we show that the distribution of products vary with the strength of the reducing agent

At this stage of purification GST-X fusion should be clean and depending on the nature of furhter experiments that we want to perform, we can either elute the fusion with glutathione (excess of the glutathione will compete and displace GST-X protein from the beads) or cleave the GST tag and release protein X (see cartoon below)

10.1053/j.gastro.2020.05.059 93 SunL.ZhaoH.LiuL.WuX.GaoQ.ZhaoY

Chinese manufacturers use similar innovations to produce pharmaceutical HGH products under the same or even higher conditions, and they are significantly more effective