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Interestingly, patients with increased intestinal permeability were also more likely to have a pathological glucose breath test, which is indicative of small intestinal bacterial overgrowth (SIBO) (88.8 vs

VWR, Rosnysous- Bois, France) in 12-well culture plates (Costar, VWR, France)

A single puncture with a small needle generates minimal particulate, but repeated draws with that same compromised stopper will progressively contaminate the solution