Before reconstitution, keep vials at -20 degrees Celsius (-4 degrees Fahrenheit) for long-term storage, or 2 to 8 degrees Celsius (35.6 to 46.4 degrees Fahrenheit) for shorter periods
Maintain structural integrity through controlled environmental parameters
Healthy follicles were identified by an intact basal lamina, a visible oocyte with a germinal vesicle, and a distinct nucleolus, while atretic follicles were characterized by pyknotic nuclei and degenerating granulosa cells.23 Immunohistochemistry The immunohistochemical staining kit (Thermo Ultravision Detection System, Fremont, USA, TP-125-HL) was used in conjunction with the streptavidin-biotin-peroxidase method
Masson's trichrome staining Masson's trichrome staining revealed minimal periventalar and periportal collagen deposition in the control, nicorandil-only, and MSC-only groups, consistent with normal hepatic architecture (Figure 4a)
Palmatine intervention effectively reduced both the number and size of these lipid droplets, with the moderate-dose group showing the most prominent reduction in hepatic steatosis
