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On the other hand, limited knowledge is known about the mechanisms of the negative effect of PSO on the expression of HSP70 and CC chemokine in tilapia sperm, and further studies should be done to clarify the suppression of these gene expressions by current PSO supplementation

[PMID: 15294021] [8] Chevolot L, Mulloy B, Ratiskol J, Foucault A, Colliec-Jouault S

However, this reduction in cytoplasm also reduces the capacity of sperm to harbor antioxidant enzymes, making them more vulnerable to oxidative stress

(Tyrosinase)

Pharmasana told me they were good for at least a year but would be less effective as time passed