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The behavioral response of the rats to the injection of a 5% formalin solution into the hind paw was used to assess pain and analgesia [9]

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Further, a near perfect correlation between mRFP-p-actin labeled spines and the general presynaptic marker Synapsin and VLGUT1 staining, which identifies excitatory glutamatergic synapses suggests that most AnglV-dependent effects on hippocampal spines were restricted to excitatory synapses

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