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The cells were collected after treatment, washed twice with cold phosphate-buffered saline, and treated with 1 mL of cold citrate buffer (0.24 M sucrose, 40 mM sodium citrate, pH 7.6)

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Handling Notes Avoid freeze-thaw cycles

These have specificities for cationic residues (arginine and lysine) and aromatic residues respectively, so that most therapeutic proteins are vulnerable to attack by one or both of these enzymes