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26, 27 Primary antibodies included anti-phospho-p65 antibody (Cell signaling #3033, 1:1000), anti-p65 antibody (Cell Signaling #6956, 1:1000), anti-IB antibody (Cell Signaling #3033, 1:1000), anti-IB antibody (Cell Signaling #9242, 1:1000), anti-phospho- IB antibody (Cell Signaling #6956, 1:2859) and anti--tubulin (Sigma-Aldrich #T-9026, 1:5000)

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This article is for general educational purposes and does not replace a medical evaluation

Estrogens are secreted cyclically, inducing a negative feedback on gonadotropins and modulating the hypothalamic-pituitary-gonadal axis (Jensen et al., 2010)