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The PCR program was set as: 98 C for 3 min, 98 C for 10 s, 5560 C (temperature and time were set according to primers), 72 C for 30 s, and a total of 35 cycles

Needleless technology significantly diminishes these events, making the treatment process safer for everyone involved

Data analysis was performed using Image-Pro Plus 6.0 software (Media Cybernetics, Inc.)

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