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B12 helps combat fatigue and may reduce nausea

Older patients may have somewhat slower clearance but the differences are usually clinically minimal

Lysed cells were scraped on dry ice and transferred to Eppendorf tubes for centrifugation at 18,000 g at 4 C for 5 min

We adjust the dose based on how well theyre doing and lab results

And then secondly, you're really hindered by the idea that you're studying that animal in an artificial environment and when you reduce the risk of a subset of death, a subset of causes of death which is effectively metabolic disease, you're often unable to measure what in my opinion is an underappreciated risk that comes on, which is, sort of, the more sudden and traumatic causes of death that we take for granted, especially in the case of caloric restriction