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After 2 h cultivation, the cells were washed with PBS and the neutral red was extracted with 0.1 M HCl30% ethanol solution

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Proteolytic action of NSPs is controlled endogenously by the serpin (serine proteinase inhibitor) superfamily of proteins (e.g., alpha1-antitrypsin (AAT), alpha1antichymotrypsin, monocyte NE inhibitor) and the chelonianin family of canonical inhibitors (elafin, secretory leukocyte protease inhibitor) (82, 83)

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