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Rest days On days without training, intake remains relevant to maintain high muscle levels and support deep recovery

Materials and methods Cell culture and transient transfection HeLa, 293FT, DU145, and H1299 cells were cultured in Dulbeccos modified Eagles medium (DMEM, WelGENE) supplemented with 10% fetal bovine serum (FBS, HyClone) and 1% penicillin-streptomycin (Gibco)