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The chromatogram itself provides visual confirmation

TB-500 is a synthetic fragment of thymosin 4 (the Ac-LKKTETQ motif), while BPC-157 is a gastric-derived pentadecapeptide

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doi:10.2174/1389557520666201127104907

The next day, samples were incubated with FITC-conjugated, TRITC-conjugated, or Cy5-conjugated (1:1000, Invitrogen, United States) secondary antibody along with 4,6-diamidino-2-phenylindole (DAPI) (1:1000, Sigma-Aldrich, United States) at room temperature for 1 h