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Bleeding Time Soluble P-Selectin Assay cGMP Assay Platelet Preparation 2 , 12 mmol/L NaHCO 3 , 0.4 mmol/L Na 2 HPO 4 , 0.35% bovine serum albumin, 10 mmol/L Hepes, 5.5 mmol/L glucose), pH 7.4, and centrifuged at 100 g for 15 minutes at 22C to obtain platelet-rich plasma

First, mutations at positions 14 and 17 stabilize the central helix through a salt bridge

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