Manual scoring of images for co-localized glucagon, GLP-1, and DAPI (Dako) was performed using ImageJ (NIH) or Photoshop (Adobe) of, at least, 9 randomly chosen fields per treatment group for each replicate
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Indeed, GLP-1 secretion is abolished upon corn oil infusion to the proximal duodenal compartment in vagotomized rats (Rocca and Brubaker, 1999)
