HBx can influence the activity of metabolic transcription factors, such as sterol regulatory element-binding proteins (SREBPs), which are key regulators of de novo lipogenesis ( Thus, for viral etiologies, the virus acts as the initial trigger, but the resulting metabolic dysregulation and chronic inflammatory state which overlaps significantly with the pathophysiology of MASLD are focused on and ultimately destroy the livers tissue architecture, creating the sustained carcinogenic environment (Wang and Zhang, 2023
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Cellular viability Optimization of Sp concentration and its cytotoxicity parameters were established by treating of 1.5 10 5 HeLa or 1.2 10 5 SHSY-5Y cells with serial concentrations of the Sp (100 M1 mM) for 15 min or conducting a time-course (5 min48 h) using 250 M Sp, followed by MTT assay as per standard protocol (Thermo Fisher Scientific)
