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Primers were designed to fit in resulting vector, pJB-HTS upstream of the hexa-histadine tag (5/phos/CCATGGGCAGCAGCCATCATCAT), and downstream (AGCTGGAATTCCTAGTTATTGCTCAGCGGTGGC) (Integrated DNA technologies) of the spacer yielding a fragment containing a phosphorylated 5 end, an initiation codon, a hexa-histadine tag, a thrombin cleavable sequence, a spacer, a termination codon, and an EcoRI site
PubMed Cheung MC, Zhao XQ, Chait A, et al
However, the specific mechanism by which changes in the gut microbiota leads to cirrhosis remains unclear
Kt hp li sng lnh mnh: n ung khoa hc, ung nc, tp th dc, ng gic gip tng hiu qu dng trng da
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