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Initial thermal-cycling at 95C for 30 s followed by 40 cycles of 95C for 3 s and 60C for 30 s

Significant changes in TCA intermediates fumarate and succinate, and in the secondary TCA metabolites succinyl-carnitine and methyl succinyl-carnitine were observed, arguing that the potential anti-inflammatory properties of these metabolites are mediated by metabolic rewiring

They will answer any questions you may have and monitor the IV setup to ensure that you remain comfortable from start to finish