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Your body is quite good at regulating B12 levels and eliminating excess through your kidneys

Its best to use one of the following areas (see Figure 1): Your abdomen (belly), except for the 2-inch (5-centimeter) area around your belly button The middle front or middle outside of your thighs The upper area of your buttocks (butt) The back part of your upper arms, if someone else is giving you the injection If your healthcare provider tells you to use a certain injection site, follow their instructions

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2.3 Western blot and antibodies The equal amounts (30 g) of protein lysed from aortic tissue and HUVECs, were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, IPVH00010)

If any, it should be minimal