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The column was then washed with 10 CV of 25 mM imidazole (Sigma-Aldrich) in DPBS (pH 7.2), followed by elution with 10 CV of 250 mM imidazole in DPBS (pH 7.3)

however, most people with NASH cannot lose weight through lifestyle interventions or sustain the weight loss achieved [86]

The weight loss seen in the trial was on par with what Novo Nordisk reported in clinical trials with people taking a weekly injection of semaglutide over the same time period

You may need to reconstitute the full amount and accept some efficiency loss