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44 Although cellular free iron concentrations are generally kept very low by binding to ferritin, iron can be mobilized from this binding protein by superoxide

The inoculated assay plates were incubated anaerobically at 37 C for 2024 h, then processed as described above under Crystal violet biofilm assay, except that the wells were not imaged prior to fixation and OD 600 readings were also not taken, as for the purposes of the high-throughput primary screen, we did not include replicate wells that could be resuspended, although we did note occasional wells with a complete failure to grow and omitted those from further analysis