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19 4.1.5'siRNAs' Table 5: siRNAs siRNA Manufacturer Catalog number ON-TARGET Plus Non- Targeting Control Dharmacon D-001210-01-05 ON-TARGET Plus CTH Dharmacon J-003481-06 MISSION siRNA Universal Negative Control #1 Sigma SIC001 MISSION siRNA FOSL1_1 Sigma SASI_Hs01_00191186 MISSION siRNA FOSL1_2 Sigma SASI_Hs02_00339593 siRNA NFE2L2_1 Ambion Assay ID: 3347 siRNA NFE2L2_2 Ambion Assay ID: 107967 4.1.6'Antibodies' Table 6: Primary antibodies for Western blot, Immunofluorescence and Immunohistochemistry Antibody Manufacturer Catalog number Applied dilution b-Actin Santa Cruz sc-47778 1:5000 ATF4 Cell Signaling 11815 1.1000/1:500 CTH Santa Cruz sc-100583 1:1000 CTH Abcam ab151769 1:500 CBS Santa Cruz sc-271886 1:1000 FOSL1 Cell Signaling 5281 1:1000 MITF C

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In 2019, with that experience under her belt, Smith felt equipped to move on to a Senior Research Associate role

doi: 10.1128/AEM.02391-12 82 MetzlerM.PfeifferE.HildebrandA

Overall, there is extensive evidence indicating that the gut microbiota has a large impact on nutrient-induced gutbrain signaling that regulates metabolic homeostasis (Fig