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The crude product was purified by a silica gel column chromatography using hexane and dichloromethane mixture (10:1 v/v) as eluent and obtained as yellow powder in 74% yield

In the heart, the metabolome is characterized by amino acid depletion, evidence of altered redox balance including glutathione depletion, and a shift from oxidative phosphorylation to alternative energy production pathways

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The SPAE 400 mg/kg group (0.90 0.05) also showed significant improvement compared to DEX alone

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