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Western blotting Total protein from lung tissues or fibroblasts was extracted with RIPA lysis buffer containing cOmplete Protease Inhibitor (Roche, Basel, Switzerland)

Weijl NI, Elsendoorn TJ, Lentjes EGWM, Hopman GD, Wipkink-Bakker A, Zwinderman AH, Cleton FJ, Osanto S

Keep the vial away from light by storing it in its original box

For most patients, the GI effects are more manageable than cardiovascular stress, but individual tolerance varies

To minimize the impact of bias, we included both male and female mice (mutants and respective gender controls) in the different experiments, as reported in the figure legends